Multiphoton Microscopy or MPM: What is it and What it Does

featured6 - Multiphoton Microscopy or MPM: What is it and What it Does

As in any other laboratory, schools around the world is currently looking at any technology that will help in the research of better ways to cure heart disease. One of the technologies they’re looking at is multiphoton microscopy or MPM. There is a lot of science that goes into the processes, but what it really is—in layman’s terms—is three-dimensional imaging or scanning of the inside of your body made better.

On the side of both photographic and medical sciences, here is what happens in the process of making multiphoton microscopy images.

In the beginning—Preparation

When you begin, you should choose carefully the technique that you’re going to use. Keep in mind that specimens that are bigger (humans or otherwise) are better if you’re using two-photon excitation process. Otherwise, you’ll need to rely on three-dimensional microscopy (fluorescence method) as an alternative to creating images for whatever need there is.

Confocal microscopy

As for this method of microscopy, it’s more a refinement of the process of multiphoton microscopy. This enables you to use a pinhole-like technique. The importance of this is to clear up the image; this process does so by using the pinhole to keep the focus on the clearer picture and keep the out-of-focus image out of detection in the picture.

Deconvolution—surveying specimens

Deconvolution provides the best out of all solutions for producing specimens. It’s a good source of creating crisp images out of low quality backgrounds, or images that are severely poor in focus. While the images produced are passable at best, the use of widefield lens keeps the excitation process at a low level. This is an effective method for use in living cells and the like.

Two-photon excitation—an old concept

The process using two-photon excitation remains your best chance for  standard crisp image. The excitation of photons leads to a good image where you can see what you need to study. It is this technique that gets more attention in experimental situations as you get more penetration no matter the depth, in contrast to other processes.

Three-photon excitation—viewing specimens

It is widely regarded a nonlinear process in the excitation of photons. It could be used in situations where three photons are needed for fluorophore reaction; the result here is that it develops images that are more useful in detection and study rather than two-photon excitation processes. While this is true, two-photon processes are still quicker to produce and is the usual standard.

Multiphoton Microscopy is a good process to study and use in the field of vascular system treatment. It provides an easier way to spot problems at the earliest stages and work on a cure or treatment for them.